Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Target K_i: 2.3 nM for protein kinase A

General description

Peptide corresponding to the active site on the skeletal muscle inhibitor protein. Competitive inhibitor of protein kinase A (K_i = 2.3 nM).

A potent synthetic peptide that is a competitive inhibitor of protein kinase A (K_i = 2.3 nM). Its sequence is derived from the heat-stable skeletal muscle inhibitor protein of PKA. Binds to the catalytic subunit of PKA and displaces the regulatory subunit. Mimics the protein substrate by binding to the catalytic site via the arginine-cluster basic substrate.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Knighton, D.R., et al. 1991. Science 253, 525.Cheng, H.C., et al. 1986. J. Biol. Chem. 261, 989.Rusine, T., et al. 1986. J. Cell Biol.102, 1630.Wong, Y.S., et al. 1986. J. Biol. Chem..261, 12089.

Packaging

Yes

500 µg in Plastic ampoule

Preparation Note

Sonication or the addition of a few drops of dilute acid (10 mM HCl) may be necessary to achieve complete solubilization.

Sequence

H-Thr-Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-His-Asp-OH

Warning

Toxicity: Standard Handling (A)